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anti-proil-1b  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti-proil-1b
    Anti Proil 1b, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti-proil-1b/anti+proil+1b/10__3390_slash_molecules25204693-263-5-6
    Average 90 stars, based on 1 article reviews
    anti-proil-1b - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: The Inhibition of NLRP3 Inflammasome and IL-6 Production by Hibiscus noldeae Baker f. Derived Constituents Provides a Link to Its Anti-Inflammatory Therapeutic Potentials
    Article Snippet: The membranes were immunoblotted by anti-ProIL-1b (Cell signaling Technology, Danvers, MA, USA) or anti-Pro caspase-1 (abcam) according to the manufacturer’s instructions.



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    (A) IL-1β, (B) IL-6, and IL-12p40 were measured by ELISA in the supernatants of WT BMDCs cultured with WT TH0 TEM in the presence or absence of anti-CD3 (200ng/mL) for 18hr. (C) IL-6 and IL-12p40 were measured by ELISA in the supernatants of WT BMDMs or CD11c+ splenic DCs cultured with WT TH0 TEM in the presence of anti-CD3 for 18hr. (D) IL-6 and IL-12p40 were measured by ELISA in the supernatants of WT or TLR2/4/5xUnc93b13d/3d BMDCs cultured with WT TH0 TEM in the presence anti-CD3 for 18hr. (E) Quantification of intracellular <t>proIL-1b</t> or IL12p40 production measured by flow cytometry of CD11c+CD11b+ or CD90.2+ cells following culture of WT BMDCs and WT TH0 TEM in the presence of anti-CD3 for 6hr. Representative flow plots shown in Fig. S1A. (F) IL-6 was measured by ELISA in the supernatants of WT or Il6−/− BMDCs cultured with WT Th0 TEM in the presence of anti-CD3 for 6hr. (G) IL-6 was measured by ELISA in the supernatants of WT BMDCs cultured with TH0 or polarized Th1, TH2, or TH17 TEM in the presence of anti-CD3 for 6hr. (H) IL-6 and IL-12p40 were measured by ELISA in the supernatants of WT BMDCs cultured with OT-II TH0 in the presence or absence of 10uM OVA peptide (323-339) (OVAp) and (I) anti-IA/IE antibody (20ug/mL) for 18hr. (J) IL-6 and IL-12p40 levels in the serum of WT mice were quantified by ELISA 6hr following injection with PBS or anti-CD3 (50ug, i.v.). Error bars indicate SEM. (A-D, F-H, J) n=3, (E, I) n= 4, (A-D,F-J) unpaired two-tailed t-test. (E) unpaired one-tailed t-test *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, ns = not significant.
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    (A) IL-1β, (B) IL-6, and IL-12p40 were measured by ELISA in the supernatants of WT BMDCs cultured with WT TH0 TEM in the presence or absence of anti-CD3 (200ng/mL) for 18hr. (C) IL-6 and IL-12p40 were measured by ELISA in the supernatants of WT BMDMs or CD11c+ splenic DCs cultured with WT TH0 TEM in the presence of anti-CD3 for 18hr. (D) IL-6 and IL-12p40 were measured by ELISA in the supernatants of WT or TLR2/4/5xUnc93b13d/3d BMDCs cultured with WT TH0 TEM in the presence anti-CD3 for 18hr. (E) Quantification of intracellular <t>proIL-1b</t> or IL12p40 production measured by flow cytometry of CD11c+CD11b+ or CD90.2+ cells following culture of WT BMDCs and WT TH0 TEM in the presence of anti-CD3 for 6hr. Representative flow plots shown in Fig. S1A. (F) IL-6 was measured by ELISA in the supernatants of WT or Il6−/− BMDCs cultured with WT Th0 TEM in the presence of anti-CD3 for 6hr. (G) IL-6 was measured by ELISA in the supernatants of WT BMDCs cultured with TH0 or polarized Th1, TH2, or TH17 TEM in the presence of anti-CD3 for 6hr. (H) IL-6 and IL-12p40 were measured by ELISA in the supernatants of WT BMDCs cultured with OT-II TH0 in the presence or absence of 10uM OVA peptide (323-339) (OVAp) and (I) anti-IA/IE antibody (20ug/mL) for 18hr. (J) IL-6 and IL-12p40 levels in the serum of WT mice were quantified by ELISA 6hr following injection with PBS or anti-CD3 (50ug, i.v.). Error bars indicate SEM. (A-D, F-H, J) n=3, (E, I) n= 4, (A-D,F-J) unpaired two-tailed t-test. (E) unpaired one-tailed t-test *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, ns = not significant.
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    (A) IL-1β, (B) IL-6, and IL-12p40 were measured by ELISA in the supernatants of WT BMDCs cultured with WT TH0 TEM in the presence or absence of anti-CD3 (200ng/mL) for 18hr. (C) IL-6 and IL-12p40 were measured by ELISA in the supernatants of WT BMDMs or CD11c+ splenic DCs cultured with WT TH0 TEM in the presence of anti-CD3 for 18hr. (D) IL-6 and IL-12p40 were measured by ELISA in the supernatants of WT or TLR2/4/5xUnc93b13d/3d BMDCs cultured with WT TH0 TEM in the presence anti-CD3 for 18hr. (E) Quantification of intracellular <t>proIL-1b</t> or IL12p40 production measured by flow cytometry of CD11c+CD11b+ or CD90.2+ cells following culture of WT BMDCs and WT TH0 TEM in the presence of anti-CD3 for 6hr. Representative flow plots shown in Fig. S1A. (F) IL-6 was measured by ELISA in the supernatants of WT or Il6−/− BMDCs cultured with WT Th0 TEM in the presence of anti-CD3 for 6hr. (G) IL-6 was measured by ELISA in the supernatants of WT BMDCs cultured with TH0 or polarized Th1, TH2, or TH17 TEM in the presence of anti-CD3 for 6hr. (H) IL-6 and IL-12p40 were measured by ELISA in the supernatants of WT BMDCs cultured with OT-II TH0 in the presence or absence of 10uM OVA peptide (323-339) (OVAp) and (I) anti-IA/IE antibody (20ug/mL) for 18hr. (J) IL-6 and IL-12p40 levels in the serum of WT mice were quantified by ELISA 6hr following injection with PBS or anti-CD3 (50ug, i.v.). Error bars indicate SEM. (A-D, F-H, J) n=3, (E, I) n= 4, (A-D,F-J) unpaired two-tailed t-test. (E) unpaired one-tailed t-test *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, ns = not significant.
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    (A) IL-1β, (B) IL-6, and IL-12p40 were measured by ELISA in the supernatants of WT BMDCs cultured with WT TH0 TEM in the presence or absence of anti-CD3 (200ng/mL) for 18hr. (C) IL-6 and IL-12p40 were measured by ELISA in the supernatants of WT BMDMs or CD11c+ splenic DCs cultured with WT TH0 TEM in the presence of anti-CD3 for 18hr. (D) IL-6 and IL-12p40 were measured by ELISA in the supernatants of WT or TLR2/4/5xUnc93b13d/3d BMDCs cultured with WT TH0 TEM in the presence anti-CD3 for 18hr. (E) Quantification of intracellular proIL-1b or IL12p40 production measured by flow cytometry of CD11c+CD11b+ or CD90.2+ cells following culture of WT BMDCs and WT TH0 TEM in the presence of anti-CD3 for 6hr. Representative flow plots shown in Fig. S1A. (F) IL-6 was measured by ELISA in the supernatants of WT or Il6−/− BMDCs cultured with WT Th0 TEM in the presence of anti-CD3 for 6hr. (G) IL-6 was measured by ELISA in the supernatants of WT BMDCs cultured with TH0 or polarized Th1, TH2, or TH17 TEM in the presence of anti-CD3 for 6hr. (H) IL-6 and IL-12p40 were measured by ELISA in the supernatants of WT BMDCs cultured with OT-II TH0 in the presence or absence of 10uM OVA peptide (323-339) (OVAp) and (I) anti-IA/IE antibody (20ug/mL) for 18hr. (J) IL-6 and IL-12p40 levels in the serum of WT mice were quantified by ELISA 6hr following injection with PBS or anti-CD3 (50ug, i.v.). Error bars indicate SEM. (A-D, F-H, J) n=3, (E, I) n= 4, (A-D,F-J) unpaired two-tailed t-test. (E) unpaired one-tailed t-test *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, ns = not significant.

    Journal: Science immunology

    Article Title: Effector Memory CD4 + T cells induce damaging innate inflammation and auto-immune pathology by engaging CD40 and TNFR on myeloid cells

    doi: 10.1126/sciimmunol.abk0182

    Figure Lengend Snippet: (A) IL-1β, (B) IL-6, and IL-12p40 were measured by ELISA in the supernatants of WT BMDCs cultured with WT TH0 TEM in the presence or absence of anti-CD3 (200ng/mL) for 18hr. (C) IL-6 and IL-12p40 were measured by ELISA in the supernatants of WT BMDMs or CD11c+ splenic DCs cultured with WT TH0 TEM in the presence of anti-CD3 for 18hr. (D) IL-6 and IL-12p40 were measured by ELISA in the supernatants of WT or TLR2/4/5xUnc93b13d/3d BMDCs cultured with WT TH0 TEM in the presence anti-CD3 for 18hr. (E) Quantification of intracellular proIL-1b or IL12p40 production measured by flow cytometry of CD11c+CD11b+ or CD90.2+ cells following culture of WT BMDCs and WT TH0 TEM in the presence of anti-CD3 for 6hr. Representative flow plots shown in Fig. S1A. (F) IL-6 was measured by ELISA in the supernatants of WT or Il6−/− BMDCs cultured with WT Th0 TEM in the presence of anti-CD3 for 6hr. (G) IL-6 was measured by ELISA in the supernatants of WT BMDCs cultured with TH0 or polarized Th1, TH2, or TH17 TEM in the presence of anti-CD3 for 6hr. (H) IL-6 and IL-12p40 were measured by ELISA in the supernatants of WT BMDCs cultured with OT-II TH0 in the presence or absence of 10uM OVA peptide (323-339) (OVAp) and (I) anti-IA/IE antibody (20ug/mL) for 18hr. (J) IL-6 and IL-12p40 levels in the serum of WT mice were quantified by ELISA 6hr following injection with PBS or anti-CD3 (50ug, i.v.). Error bars indicate SEM. (A-D, F-H, J) n=3, (E, I) n= 4, (A-D,F-J) unpaired two-tailed t-test. (E) unpaired one-tailed t-test *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, ns = not significant.

    Article Snippet: Cells were immediately harvested on ice, stained with fixable Zombie Yellow (Biolegend), then fixed with Foxp3 Transcription Factor Staining Set (Invitrogen) and stained with anti-mouse proIL-1b (eBioscience, NJTEN3) or anti-mouse IL-12/23 p40 (Biolegend, C15.6) according to manufacturer protocol.

    Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Flow Cytometry, Injection, Two Tailed Test, One-tailed Test